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李荣凤

李荣凤，博士，教授，博士生导师

地址：南京医科大学先知楼2105

电话：025-86862122

E-mail：lirf01@126.com

 
 
李荣凤，女，满族。1992年毕业于内蒙古大学生物系，同年留在内蒙古大学实验动物研究中心工作。分别于1997和2003年获得理学硕士和博士学位，导师旭日干院士。1995年至1996年，赴日本农林水产省开展国际合作研究。2005年至2007年，在美国密苏里大学做博士后研究。目前为南京医科大学代谢疾病研究中心特聘教授，博士生导师。参加工作以来一直从事家畜体外受精、细胞核移植、转基因克隆动物和哺乳动物胚胎干细胞等研究工作。在主持内蒙古自治区自然科学基金项目“体细胞核移植技术在优质高产奶牛快速繁育中的应用”过程中，培育出两头健康的体细胞克隆牛。 在密苏里大学期间，作为主要完成人之一，成功获得了hfat-1转基因猪。作为第一作者于2006年利用显微操作去脂法培育出世界首批经胚胎冷冻保存的体细胞克隆转基因猪。在此基础上，发明了一种无需显微操作的早期胚胎去脂方法，培育了世界第一批非显微去脂法冷冻保存的体外受精猪。申请国际专利一项（US61/190,515）。发表学术论文30余篇，其中19篇为SCI收录。作为副主编出版专著一部《家畜性别控制技术》（科学出版社，2009）。主持研究的成果“哺乳动物胚胎低温耐受性机理和冷冻保存技术研究”于2009年获得内蒙古自治区自然科学奖二等奖。
 
 

主要研究方向：

 
1、哺乳动物体细胞克隆
 
2、转基因猪疾病动物模型的研究
 
3、异种组织和器官移植用供体-转基因克隆猪的研制
 

4、哺乳动物胚胎干细胞研究

 

 

承担项目：

 

国家自然科学基金“利用iPSCs技术辅助建立猪Naïve胚胎干细胞系及Naïve与Primed胚胎干细胞转化机制研究”（31371522），负责人，正在进行

 

 

近年发表的主要学术论文

 

1.  Liu Q, Zhang ML, Hou DX, Han XJ, Jin Y, Zhao LH, Nie XW, Zhou X, Yun T, Yuhang Zhao YH, Huang XH, DR,2, Yang N, Wu ZQ, Li XL, Li RF. 2014. Karyotype Characterization of In Vivo- and In Vitro- Derived Porcine Parthenogenetic Cell Lines. PLOS ONE. 9(5): e97974

2.  Huang XH, Han XJ, Uyunbilig B, Zhang ML, Duo SG, Zuo YC, Zhao YH, Yun T, Tai DT, Wang C, Li JH, Li XL, Li RF. 2014. Establishment of Bovine Trophoblast Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos Using Two Inhibitors. STEM CELLS AND DEVELOPMENT. 23(13): 1501-1514

3.  Isom C, Li R, Whitworth KM, Prather RS.2012, Timing of First Embryonic Cleavage Is a Positive Indicator of the In Vitro Developmental Potential of Porcine Embryos Derived From In Vitro Fertilization, Somatic Cell Nuclear Transfer and Parthenogenesis. Molecular Reproduction and Development 79:197

4.  Whitworth KM, Zhao J, Spate LD, Li R, Prather RS. 2011. Scriptaid corrects gene expression of a few aberrantly reprogrammed transcripts in nuclear transfer pig blastocyst stage embryos. Cellular Reprogramming.13(3):191-204.

5.  Whitworth KM, Spate LD, Li R, Rieke A, Sutovsky P, Green JA, Prather RS. 2010. Activation method does not alter abnormal placental gene expression and development in cloned pigs. Molecular Reproduction and Development 77:1016-1030.

6.  Whitworth KM, Li R, Spate LD, Wax DM, Rieke A, Whyte JJ, Manandhar G, Sutovsky M, Green JA, Sutovsky P, Prather RS. 2009, Method of Oocyte Activation Affects Cloning Efficiency in Pigs. Mol. Reprod. Dev. 76(5):490-500。

7.  Li R, Murphy CN, Spate L, Wax D, Isom C, Rieke A, Walters E, Samuel M, Prather RS. 2009, Production of Piglets after Cryopreservation of Embryos Using a Centrifugation-Based Method for Delipation Without Micromanipulation. Biol Reprod.80:563-571.

8.  Zhong Z, Hao Y, Li R, Spate L, Wax D, Sun QY, Prather RS, Schatten H. 2008, Analysis of heterogeneous mitochondria distribution in somatic cell nuclear transfer porcine embryos. Microscopy Microanalysis. 14(5):418-32.

9.  Antelman J, Manandhar G, Yi Y-J, Li R, Whitworth KM, Sutovsky M, Agca C, Prather RS, Sutovsky P. 2008, Expression of Mitochondrial transcription factor A(TFAM) during porcine gametogenesis and preimplantation embryo development. Journal of Cellular Physiology. 217(2):529-543.

10.  McHughes CE, Springer GK, Spate L, Li R, Woods RJ, Green M, Korte SW, Murphy CN, Green JA, Prather RS.2009,Identification and quantification of differentially represented transcripts in in vitro and in vivo derived preimplantation bovine embryos, Mol. Reprod. Dev. 76(1):48-60.

11.  Bonk AJ, Cheong HT, Li R, Lai L, Hao Y, Liu Z, Samuel M, Fergason EA, Whitworth KM, Murphy CN, Antoniou E, Prather RS. 2007, Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Epigenetics, 2(3), 179-186.

12.  Yi YJ, Mananadhar G, Sutovsky M, Li R, Jonakova V, Oko R, Park CS, Prather RS, Sutovsky P. 2007, Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod. 77(5), 780-793.

13.  Bonk AJ, Li R, Lai L, Hao Y, Liu Z, Samuel M, Ferguson EA, Whitworth KM, Murphy CN, Antoniou E, Prather RS. 2008, Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced embryos. Mol. Reprod. Dev. 75(2), 250-264.

14.  Zhong z, Spate L, Hao Y, Li R, Lai L, Katayama M, Wax D, Whitworth K, Sun QY, Prather RS, Schatten H. 2007. Remodeling of centrosomes in intraspecies and interspecies nuclear transfer porcine embryos. Cell Cycle. 6(12), 1510-1520.

15.  Li R, Whitworth K, Lai L, Wax D, Spate L, Murphy CN, Rieke A, Isom C, Hao Y, Zhong Z. Katayama M, Schatten H, Prather RS. 2007. Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos. Mol. Reprod. Dev. 74(9), 1228-1235.

16.  Li R, Lai L, Wax D, Hao Y, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Turk JR, Kang JX, Witt WT, Dai Y and Prather RS. 2006. Cloned transgenic swine via in vitro production and cryopreservation. Biol. Reprod. 75, 226-230.

17.  Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, Hao Y, Wax DM, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Starzl TE, Prather RS and Dai Y. 2006. Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nature Biotechnology, 24(4), 435-436.

18.  Yong H.Y., Hao Y, Lai L, Li R, Murphy CN, Rieke A, Wax D, Samuel M and Prather RS. 2006. Production of a transgenic piglet by a sperm injection technique in which no chemical or physical treatments were used for oocytes or sperm. Mol. Reprod. Dev. 73, 595-599.

19.  Li R, Wen L, Wang S, Bou S. 2006. Development, freezability and amino acids consumption of bovine embryos cultured in synthetic oviductal fluid (SOF) medium containing amino acids at oviductal or uterine fluid concentrations. Theriogenology, 66, 404-414.